snap surface af647  (New England Biolabs)

Journal: bioRxiv

Article Title: High Refractive Index Imaging Buffer for Dual Color 3D SMLM Imaging of Thick Samples

doi: 10.1101/2024.04.30.591648

Figure Lengend Snippet:

Article Snippet: We labeled Nup96-SNAP with SNAP-tag ligand O 6 -benzylguanine conjugated AF647 (BG-AF647, S9136S, New England Biolabs) as described else ( ).

Techniques:

Journal: bioRxiv

Article Title: High Refractive Index Imaging Buffer for Dual Color 3D SMLM Imaging of Thick Samples

doi: 10.1101/2024.04.30.591648

Figure Lengend Snippet: (a-c) Representative images of microtubules (anti-β-tubulin/CF680) and the outer mitochondrial membrane (anti- Tom20/AF647) in CUBIC-R+ (a), 3-PM (b) and WI (c) buffers. Color bar denotes the z position. (d) The side view of the white dashed regions in (a-c). The white arrows indicate the mitochondria-microtubule contacts, while the red arrows denote the different layers of microtubule filaments. (e, f) FRC curves of microtubule (e) or mitochondria (f) in regions corresponding to the bottom panels of (a-c). Scale bars: 2 μm (a-c), 200 nm (d).

Article Snippet: We labeled Nup96-SNAP with SNAP-tag ligand O 6 -benzylguanine conjugated AF647 (BG-AF647, S9136S, New England Biolabs) as described else ( ).

Techniques: Membrane

Journal: bioRxiv

Article Title: High Refractive Index Imaging Buffer for Dual Color 3D SMLM Imaging of Thick Samples

doi: 10.1101/2024.04.30.591648

Figure Lengend Snippet: (a, c) Dual-color 3D SMLM images of microtubules (anti-β-tubulin/CF680) and the outer mitochondrial membrane (anti-Tom20/AF647) in CUBIC-R+ (a) and WI (c) buffers. Color bars denote the z position. (b, d) The side view of the white dashed regions in (a, c). The white arrows indicate the mitochondria-microtubule contacts, while the red arrows denote the different layers of microtubule filaments. Scale bars: 2 μm (a, c), 200 nm (b, d).

Article Snippet: We labeled Nup96-SNAP with SNAP-tag ligand O 6 -benzylguanine conjugated AF647 (BG-AF647, S9136S, New England Biolabs) as described else ( ).

Techniques: Membrane

Journal: bioRxiv

Article Title: High Refractive Index Imaging Buffer for Dual Color 3D SMLM Imaging of Thick Samples

doi: 10.1101/2024.04.30.591648

Figure Lengend Snippet: (a) Three mouse brain tissue slices (30 μm thick) were imbedded in CUBIC-R+, 3-PM and PBS for 10 min before taking photos, respectively. (b) Diagram of experimental setup for deep imaging of NPCs. (c, d) 3D SMLM images of Nup96 labeled by AF647 in CUBIC-R+ (c) and 3-PM (d) buffers. The right panels show zoomed images for regions in white, and the color bar show the z position. (e) The side view images of the white dashed regions in (c, d). (f) Plots of distance between the rings versus the z position for Nup96 in different depths and buffers. (g) Scatter plots of the photon number (left) and ELE (right) for Nup96 in oil-index buffers. Each symbol corresponds to one cell nucleus. Horizontal bars and errors show the means and SD, respectively. Scale bars: 2 μm (c, d), 200 nm (e).

Article Snippet: We labeled Nup96-SNAP with SNAP-tag ligand O 6 -benzylguanine conjugated AF647 (BG-AF647, S9136S, New England Biolabs) as described else ( ).

Techniques: Imaging, Labeling